Email: [email protected]
tel: +8618221755073
Email: [email protected]tel: +8618221755073
Mutation of gyrA was detected in 93.1% (27/29) and (21/21) among the resistant strains in both periods, respectively. A novel Gly-85 mutation of gyrA was found and confirmed to be associated with fluoroquinolone resistance. Fluoroquinolone resistance rate of H. pylori has markedly increased over time in Korea. The resistance is …
Daha fazla öğrenin
In many CIP-resistant gyrA mutants, residue 83 is replaced by a bulkier, non-polar residue that is thought to impede DNA-binding to gyrase, and hence, also fluoroquinolone binding. Interestingly in E. coli, residue 83 is a serine, whereas in P. aeruginosa it is the bulkier threonine; this may correlate with the higher intrinsic …
Daha fazla öğrenin
Mutations in gyrA were found at positions 83 and 87 of the quinolone resistance determining regions (QRDRs). 4 amino acid substitutions were seen in gyrA including double mutations at codons 83 ...
Daha fazla öğrenin
Quinolone resistance is increasing in Neisseria meningitidis, with its prevalence in China being high (>70%), but its origin remains unknown.The aim of this study was to investigate the donors of mutation-harboring gyrA alleles in N. meningitidis A total of 198 N. meningitidis isolates and 293 commensal Neisseria isolates were collected …
Daha fazla öğrenin
Whereas DNA gyrase ATPase inhibition experiments, DNA gyrase supercoiling assays, and in vitro antibacterial assays suggest binding of the hybrids to the E. coli GyrA and GyrB subunits, an interaction with the GyrA fluoroquinolone-binding site seems to be solely responsible for their antibacterial activity.
Daha fazla öğrenin
We conducted a systematic review of studies comparing N. gonorrhoeae gyrA genotype results with conventional antimicrobial susceptibility testing results. We identified 31 studies meeting inclusion criteria, among which 7 different loci for mutations in the gyrA gene were identified, from 16 countries between the years of 1996 and 2016. ...
Daha fazla öğrenin
The gyrA gene had a predicted open reading frame (ORF) encoding 878 amino acids, preceded by putative promoter and ribosome binding sequences (data not shown). The …
Daha fazla öğrenin
Menzel, R., M. Gellert 1987. Fusions of the Escherichia coli gyrA and gyrB control regions to the galactokinase gene are inducible by coumermycin treatment. J.Bacteriol. 169:1272-1278; Hussain, K., E.J. Elliot, G.P.C. Salmond 1987. The parD- mutant of Escherichia coli also carries a gyrA_Am mutation. The complete sequence of gyrA. Mol.Microbiol ...
Daha fazla öğrenin
Mutations in gyrA were absent, however, when 43 mutants with decreased ciprofloxacin susceptibility were selected from J53(pMG252) with qnrA1. Instead, in 13 mutants, individual and whole-genome sequencing identified mutations in marR and soxR associated with increased expression of marA and soxS and, through them, increased …
Daha fazla öğrenin
The sensitivity and specificity of qPCR of 23S rRNA gene were and 98.7%, respectively, while and 99.8% for qPCR of gyrA, respectively. Conclusion: The effectiveness of this qPCR is that it is sensitive in detecting low bacterial load and will help in timely detection of clarithromycin- and levofloxacin-resistant strains, especially in ...
Daha fazla öğrenin
DNA gyrase is an A2B2 heterotetramer, comprised of two subunits, that is, A (encoded by gyrA, 97 KDa in E-coli) and B (encoded by gyrB, 90 KDa in E-coli ). It has three domains like GyrB, GyrA, and C-terminal domain. GyrB belonging to GHKL family …
Daha fazla öğrenin
Four hundred sixty-two nalidixic acid- and/or ciprofloxacin-resistant Salmonella isolates were examined for presence of quinolone-resistance mechanisms. A total of 339 amino acid substitutions were identified in GyrA (204) and ParC (135). Ser83Phe/Asp87Gly (29.4%) were most commonly detected in GyrA …
Daha fazla öğrenin
The gyrA mutation interacts with the most frequent Campylobacter drug efflux pump, CmeABC, to promote the development of ciprofloxacin-resistant bacteria when its expression is raised (116, 117). Overexpression of the CmeABC efflux pump does not result in ciprofloxacin resistance without the gyrA gene mutation (114, 117, 118).
Daha fazla öğrenin
Amplification of gyrA and parC genes yielded PCR products of 349 and 327 bp, respectively (Figure 1). The nucleotide sequencing results revealed that 45 (90%) of the 50 isolates had amino acid alteration in gyrA and parC, as follow: 1 (2.2%) isolate in gyrA only, 2 (4.4%) isolates in parC only, and 42 (93.3%) isolates in gyrA and parC ...
Daha fazla öğrenin
The studies that investigated the molecular basis of FQ resistance of M. leprae were mainly based on M. leprae gene sequences [13, [24], [25], [26]], and rarely on E. coli sequences [27, 28].Since the QRDR of GyrA is located at the N-terminal part of the GyrA subunit and the M. leprae gyrA start codon is eight and one codon(s) upstream of …
Daha fazla öğrenin
The gyrA gene of the Bacillus genus shows higher variation rates than 16S rRNA. The housekeeping gene gyrA is considered to be more variable than 16S rRNA …
Daha fazla öğrenin
yüksek kalite 90kw Plastik Atık Öğütücü Kırma Su Kırılabilir PVC Borular Çin'den, Çin lider 90kw plastik geri dönüşüm değirmeni Ürün, sıkı kalite kontrol ile 1200mm plastik geri dönüşüm değirmeni fabrikalar, yüksek kalite üretmek 1200mm plastik geri dönüşüm değirmeni Ürünler.
Daha fazla öğrenin
DNA gyrase, a ubiquitous bacterial enzyme, is a type IIA topoisomerase formed by heterotetramerisation of 2 GyrA subunits and 2 GyrB subunits, to form the active complex. GyrA is usually found as a dimer in solution, whereas GyrB can exist as a monomer. DNA gyrase is able to loop DNA around the C-terminal domains (CTDs) of …
Daha fazla öğrenin
The gyrA gene of the Bacillus genus shows higher variation rates than 16S rRNA. The housekeeping gene gyrA is considered to be more variable than 16S rRNA and has been used as a molecular tool for the classification and identification of B. subtilis species (Chun and Bae 2000; Borshchevskaya et al. 2013).In the genus Bacillus, the …
Daha fazla öğrenin
Cryo-EM 3D reconstruction and molecular model of the DNA gyrase complex. a Schematic representation of the GyrA and GyrB domains, the position of the conserved catalytic tyrosine in the DNA ...
Daha fazla öğrenin
GyrA dimers eluted from the column in a single, defined peak at 9.7 ml. The elution volume was increased to 11 ml for GyrA lacking the CTDs (A ΔCTD ·A ΔCTD), consistent with its lower molecular mass. Heterodimeric A·A ΔCTD eluted as a broader peak, with a maximum at an elution volume of 10 ml.
Daha fazla öğrenin
DNA gyrase is a type II topoisomerase that introduces or removes negative supercoils, forms or resolves catenanes, and knots or unknots DNA (Gellert et al., 1976; Kreuzer …
Daha fazla öğrenin
A GyrA dimerization interface (the "C-gate"), which is formed by a pair of coiled-coil arms extending from the principal DNA binding platform, was closed in the moxifloxacin-bound complex (Fig. 2 C and D); this region has been seen in different conformational states in other type IIA topoisomerase structures (41, 42).
Daha fazla öğrenin
The role of DNA gyrase in F'lac plasmid conjugation was studied using Escherichia coli gyrA43 (Ts), gyrB41(Ts), and dnaA46(Ts) thermosensitive mutants as donor or recipient organisms, and a rifampicin or nalidixic acid-resistant J-53 strain in the presence or absence of nalidixic acid. Mating experiments were also performed employing Hfr …
Daha fazla öğrenin
List of 12 best GYRA meaning forms based on popularity. Most common GYRA abbreviation full forms updated in August 2023. Suggest. GYRA Meaning. What does GYRA mean as an abbreviation? 12 popular meanings of GYRA abbreviation: 5 Categories. Sort. GYRA Meaning 2. gyrA. Gyrase. Microbiology. Microbiology. 2. GyrA. gyrase A …
Daha fazla öğrenin
Aşağıdaki ayarlar mevcuttur: P0010 = 0: Hazır. Dönüştürücüyü başlatmak için P0010'un 0'a ayarlanması gerekir. P0010 = 1: Hızlı devreye alma. P0010 = 2: Güç ünitesi başlatma. P0010 = 3: Motor başlatma. P0010 = 4: Enkoder başlatma. P0010 = 5: Teknolojik uygulama/birimler. P0010 = 11: Fonksiyon modülleri.
Daha fazla öğrenin
The activity of gyrase. (A) DNA gyrase catalytic cycle.(B) Replication introduces (+) supercoils ahead and precatenated DNA behind.Gyrase acts ahead of the fork while topo IV removes precatenanes behind. (C) Gyrase removes (+) supercoiling from ahead of RNAP to ensure unperturbed transcription.(D) Time course supercoiling assays …
Daha fazla öğrenin
RFLP was used to molecular analysis of quinolones or fluoroquinolone resistant K. pneumoniae strains. To detect the mutation in the gyrA, PCR products of gyrA were digested by HinfI restriction enzyme. Generally the target gyrA gene contains HinfI restriction sites that one of them is located at the codon 83 serine.
Daha fazla öğrenin
Abstract. Four amino acid substitutions, two in GyrA and two in ParC subunits of DNA gyrase and topoisomerase IV, respectively, are commonly responsible for fluoroquinolone resistance in Escherichia coli.In this study, an economical and time-efficient mismatch amplification mutation assay (MAMA) PCR was developed to detect mutations …
Daha fazla öğrenin
WhatsApp24H Online Service Copyright ©2000- 2023 SHANGHAI ZENITH MINERALS CO.,LTD.
